Antiproliferative activity and apoptosis induced
by 6-bromo-2-(morpholin-1-yl)-4-anilinoquinazoline on cells of leukemia lines
S. Jantova1, A. Repicky1, E. Paulovicova2, S.
Letasiova1, L. Cipak3, *
1Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical
and Food Technology, Slovak University of Technology, Bratislava, Slovakia 2Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia 3Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia
Abstract. Quinazolines are known to be multitarget agents with broad spectrum of biological
activity. Aim: To investigate anticancer activity of newly prepared
6-bromo-2-(morpholin-1-yl)-4-anilinoquinazoline (BMAQ) towards L1210, HL-60 and U-937
leukemia cells. Materials and Methods: Growth inhibition of BMAQ-treated cells was
determined by cell counting using trypan blue staining technique. Apoptosis and cell cycle
profile changes were analysed using internucleosomal DNA fragmentation assay, fluorescence
microscopy and flow cytometry. Activity of caspase-3 was determined using colorimetric
method. Results: Cell proliferation assay showed that BMAQ caused significant decrease of
cell number in a dose-dependent manner. BMAQ induced cell death by apoptosis, based on
results from DNA fragmentation, fluorescence microscopy and caspase-3 assays. Conclusion:
Presented results clearly demonstrate that BMAQ is a promising anticancer agent with
significant antiproliferative and apoptotic activities towards leukemia cells in vitro.
Key Words:quinazoline, apoptosis, leukemia.
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