Vol. 27, No. 2, 2005 (June)
	Content

EXPRESSION OF BETA-DEFENSIN-2 IN HUMAN GASTRIC TUMORS: A PILOT STUDY

Natalya Markeeva¹, Igor Lisovskiy¹, Valeriy Lyzogubov^1,3, Vasilij Usenko³, Maria Soldatkina¹, Serhey Merentsev¹, Serhey Zaitsev², Yuriy Kondratskii², Anatoliy Tofan², Serhey Osinskiy¹, Petro Pogrebnoy¹

¹R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kyiv, Ukraine
²Institute of Oncology AMS of Ukraine, Kyiv, Ukraine
³Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine

Abstract. The present work was aimed on the study of patterns of human beta-defensin-2 (hBD-2) expression in human gastric tumors. Materials and methods: 17 samples of surgically resected human gastric adenocarcinoma paired with respective normal tissue controls have been analyzed for the expression of hBD-2 by semi-quantitative RT-PCR and by immunohistochemical analysis using anti-hBD-2 monoclonal antibodies (mAbs) generated against recombinant hBD-2 by standard hybridoma technology. Results: The hyperexpression of hBD-2 on mRNA and protein levels was registered in 6 from 17 gastric tumor samples compared to respective controls; however, hBD-2 expression patterns seems not be related to the differentiation grade and stage of tumors and the presence of anti-Helicobacter pylori-antibodies in the blood serum of the patients. Conclusion: In human gastric adenocarcinomas expression of hBDs possesses heterogeneic character; the research on larger cohort of the patients with gastric cancer should be further performed.

Key Words: human beta-defensin 2, gastric tumor, expression, monoclonal antibody.

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