Vol. 27, No. 1, 2005 (March)
	Content

AN IN VITRO STUDY OF CYTOTOXIC EFFECTS OF GOSSYPOL ON HUMAN EPIDERMOID LARYNX CARCINOMA CELL LINE (HEp-2)

Ece Konac¹, Abdullah Ekmekci¹, Erkan Yurtcu², Mehmet Ali Ergun³

¹Gazi University, Faculty of Medicine, Department of Medical Biology and Genetics, Besevler, Ankara, Turkey
²Gazi University, Faculty of Medicine, Hematology Laboratory, Besevler, Ankara, Turkey
³Gazi University, Faculty of Medicine, Department of Medical Genetics, Besevler, Ankara, Turkey

Abstract. Aim: The study was aimed on an evaluation of the effect of gossypol at concentration of 25–150 mM on the level of apoptosis in the human epidermoid larynx carcinoma cell line, HEp-2, in vitro. Methods: To determine the apoptotic effects of gossypol on growth of human epidermoid larynx carcinoma cell lines (HEp-2 cells), morphological evaluation of the cells and DNA fragmentation analysis were used. Results: 6 h incubation of HEp-2 cells with gossypol at concentrations of 50 mM and 75 mM resulted in the increase of apoptosis rate by 26 and 23%, respectively. Conclusion: This preliminary study may constitute a base for in vivo studies of anticancer properties of gossypol.

Key Words: HEp-2 cell line, gossypol, apoptosis, DNA fragmentation.

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