Vol. 27, No. 1, 2005 (March)
	Content

2-[(3-CARBOXY-1-OXOPROGY1) AMINO]-2-DEOXY-D-GLUCOSE INDUCES APOPTOSIS IN Hep G2 CELLS

Jing Wu^1,2, Delu Qi³, Kou Wei⁴, Juan Li⁵, Xuan Chen⁵, Aiqing Wang², Weiming Liu², Qunji Xue², Liang Qiao^1,6

¹Department of Gastroenterology and Hepatology, the First Hospital of Lanzhou University, Lanzhou 730000, China
²Lanzhou Institute of Chemical Physics, Chinese Academia of Sciences, Lanzhou 730000, China
³Department of Surgery, People’s Hospital of Lintao County, Lintao 730500, Gansu Province, China
⁴Medical College of Northwest Minorities University, Lanzhou 730030, China
⁵Central laboratory, the First Hospital of Lanzhou University, Lanzhou 730000, China
⁶Storr Liver Unit, Westmead Millennium Institute, Department of Gastroenterology and Hepatology, University of Sydney at Westmead Hospital, Westmead, NSW 2145, Australia

Abstract. Aim: To determine the effect of 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose (COPADG) on proliferation and apoptosis of human hepatocellular carcinoma cells (Hep G2). Methods: Hep G2 cells were cultured in RPMI-1640 medium in the presence of various concentrations of COPADG. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy, transmission electron microscopy, agarose gel electrophoresis of DNA fragmentation, and flow cytometry. Results: At the concentration ranging between 1–30 mM, COPADG potently inhibits growth and induce apoptosis of Hep G2 cells. Conclusions: COPADG could effectively induce apoptosis in Hep G2 cells. It may be potentially useful as a new agent for treatment of human hepatocellular carcinoma.

Key Words: apoptosis, hepatoma, Hep G2, 2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-glucose, COPADG.

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