Vol. 26, No. 4, 2004 (December)
	Content

CATALYTIC SYSTEM OF THE REACTIVE OXYGEN SPECIES ON THE C60 FULLERENE BASIS

Аnatolij P. Burlaka¹, Yevgeniy P. Sidorik¹, Svitlana V. Prylutska², Оlga P. Маtyshevska², Olexandr А. Golub², Yurij I. Prylutskyy², Peter Scharff³

¹R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
²National T.G. Shevchenko University, Кyiv, Ukraine
³Institute of Physics, Technical University of Ilmenau, Ilmenau, Germany

Abstract. Aim: To study the ability of fullerenes C₆₀ to catalise the reactions of generation of reactive oxygen species (ROS) in water solution after photoexcitation and to affect the vitality of tumor cells in vitro. Methods: The number and vitality of cultured Ehrlich carcinoma cells or rat thymocytes were determined using tripane blue, ROS levels were registered using the methods of electron paramagnetic resonance (EPR) spectroscopy and spin traps, photoirradiation of water solution of fullerenes С₆₀ with visible light was carried out using mercury lamp. Results: Irradiation of water solution of fullerenes С₆₀ (10^-5 М) was accompanied with generation of ROS with the rate of 10 nMol/ml/min. After addition of irradiated С₆₀ solution to suspension of thymocytes or ascite cells, the decrease of the number of vital cells by 67 and 58%, respectively, has been registered. Conclusion: Photoirradiated water solutions of fullerenes С₆₀ may be potentially useful for photodynamic therapy of tumors as ROS catalytic system.

Key Words: aqueous C₆₀ fullerene solution, irradiation by visible light, ROS, anticancer therapy, spin trap, EPR spectroscopy.

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