Vol. 26, No. 4, 2004 (December)
	Content

5-AMINOLEVULINIC ACID MEDIATED PHOTODYNAMIC THERAPY OF LEWIS LUNG CARCINOMA: A ROLE OF TUMOR INFILTRATION WITH DIFFERENT CELLS OF IMMUNE SYSTEM

Larisa M. Skivka¹, Oksana B. Gorobets², Vadim V. Kutsenok², Miron O. Lozinsky³,

¹T.G. Shevchenko Kyiv National University, Kyiv, Ukraine
²Kavetsky Institute for Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kyiv, Ukraine
³Institute of Organic Chemistry, NAS of Ukraine;
⁴“Photonics plus”, Ukraine

Abstract. Aim: To investigate the effect of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) as a photosensitizer on the character of infiltration of experimental tumors by different cells of immune system. Methods: The effect of ALA-PDT on subcutaneously implanted Lewis lung carcinoma in с57Вl/6 mice was studied. ALA at a dose of 500 mg/kg was given per os, and 4 h later tumors were subjected to laser irradiation (632 nm, 150 mW/cm², 20 min). The evaluation of the tumor infiltration by myelomonocytic and lymphoid cells and oxygen-dependent metabolism of peritoneal macrophages (NBT-test) were carried out. Results: It is shown that ALA-PDT resulted in fast and massive infiltration of irradiated tumors by myelomonocytic cells, stimulation of the peritoneal macrophages metabolic activity and augmentation of the content of tumor infiltrating lymphocytes. Conclusion: The immunomodulation after ALA-PDT occurs via generation of inflammation and direct laser light activation of immune system cells.

Key Words: helium-neon laser, photodynamic therapy, 5-aminolevulinic acid, Lewis lung carcinoma, infiltration of tumor with cells of immune system.

Language:

[full text]