STAGE OF DIFFERENTIATION, PROLIFERATIVE INDEX OF TUMOR CELLS AND CYTOTOXIC ACTIVITY OF PERIPHERAL BLOOD LYMPHOCYTES IN COLORECTAL CANCER PATIENTS
Tatyana D. Paholyuk1, Lubov M. Zacharzeva2, Konstantin V. Koshel'2, Gennadiy P. Oliynichenko2, Ninel M. Berezhnaya1
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiology NAS of Ukraine, Kyiv, Ukraine 2Kyiv Municipal Oncological Hospital, Kyiv, Ukraine
Abstract. Aim: To evaluate prognostic value of activity of cytotoxic lymphocytes of peripheral blood (CTL) of patients with colorectal cancer (CRC). Methods: 2-year survival of 30 patients with colorectal cancer stage II (T2–3N0–1M0G1–3) was investigated in relation with clinical data, cytotoxic activity of peripheral blood lymphoñytes (by ÌÒÒ-test) and immunohistochemical peculiarities of tumor cells using anti-Ki-67-MABs. Results: It was revealed that some factors had prognostic significance, namely regional lymph nodes involvement (r = –0.69), proliferative activity of tumor cells (Ki-67-positivity) (r = –0.67), restoration of CTL function after operation (r = 0.46), adjuvant chemotherapy (r = 0.41) and differentiation stage of tumor (r = 0.40). Negative correlation between CTL activity before operation and tumor proliferative activity also exists (r = –0.35). Conclusion: the data point to the involvement of natural killer cells in the control of tumor growth.
