SEA-BUCKTHORN JUICE PROTECTS MICE AGAINST GENOTOXIC ACTION OF CISPLATIN
Armen Nersesyan1, Rafael Muradyan2
Cancer Research Centre, Yerevan, Armenia1 Institute of Fine Organic Chemistry, Yerevan, Armenia2
Abstract. Aim: To study the influence of sea-buckthorn (SB) juice on the micronucleus (MN) frequency in bone marrow cells and sperm abnormality induced by cisplatin (CP, cis-dichlordiammineplatinum-II). Materials and methods: The experiments were performed with male Swiss albino mice. SB juice (0.3 ml) prepared ex tempore was given to mice by gavage during 5 or 10 days. 3 h after the last gavage, mice received CP at doses either 1.2 mg/kg or 2.4 mg/kg i.p. MN frequency was studied in bone marrow polychromatic erythrocytes 24 h after the injection of the drug. The abnormality of sperm heads was studied by microscopy. Results: The SB juice decreased significantly the number of MN in bone marrow cells induced by CP at dose of 1.2 mg/kg by 36.5% and 47.9% (when it was given 5 and 10 days, consequently), and by 19.0% (p > 0.05) at dose of 2.4 mg/kg. The SB juice decreased significantly also the damaging effect of CP on sperm head at low dose and not significantly at the higher one. Antigenotoxic effect of juice was 45.0% and 16.6%, respectively. Conclusion: SB juice decreased significantly the genotoxic effect of CP at dose of 1.2 mg/kg on somatic (bone marrow) and germ (sperm) cells of mice. At higher dose of the drug the effects was not statistically significant.
