Vol. 26, No. 2, 2004 (June)
	Content

ANTISERA TO POLYCYCLIC AROMATIC HYDROCARBONS AND THEIR APPLICATION ON THE DETECTION OF CHEMICAL CARCINOGENS IN BLOOD SERA OF ONCOLOGICAL PATIENTS

Andrey N. Glushkov¹, Tatiana P. Anosova¹, Mikhail V. Kostyanko², Mikhail P. Anosov¹, Elena G. Polenok¹, Sergey V. Cherno¹, Stella A. Mun¹

¹Kemerovo Scientific Centre, Siberian Branch of Russian Academy of Sciences, Kemerovo 650610, Russia
²Kemerovo State University, Kemerovo 650043, Russia

Abstract. Aim: To investigate the cross-reactivity of the hyperimmune antisera of animals (rabbits) and the sera of oncological patients to polycyclic aromatic hydrocarbons (PAHs) of a similar chemical structure. Methods: Reactions of antibodies with haptens have been estimated by non-competitive and competitive ELISA with synthesized protein conjugates of PAHs as a coating antigene. Results: All the model rabbit antisera have been stated to react with anthracene, chrysene, pyrene, benzo(a)pyrene and benz(a)anthracene irrespective of the hapten used to immunize animals. The cross-reactivity of serum antibodies to all five PAHs has been found in blood samples of oncological patients. Conclusion: It is recommended to use the conjugates of anthracene, chrysene and pyrene for detecting human antibodies to more dangerous environmental carcinogens — benzo(a)pyrene and benz(a)anthracene.

Key Words: cross-reactivity, antibodies, polycyclic aromatic hydrocarbons (PAHs), protein conjugates.

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