DETECTION OF POLYOMAVIRUS DNA IN HUMAN BRAIN TUMORS
Iryna G. Vasilyeva*, Mikhail I. Shamaev, Alexandr Ya. Glavatskiy, Natalya G. Chopick, Natalya P. Olexenko, Olga I. Tsyubko, Elena S. Galanta, Tatyana A. Malisheva
A.P. Romodanov Institute of Neurosurgery of Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
Abstract. Aim: to investigate surgically resected human brain tumors for the presence of polyomavirus DNA. Materials and Methods: 65 samples of surgically resected human brain tumors (57 tumors were of neuroectodermal genesis, 4 — mesenchimal, 4 — metastatic tumors) were examined for the presence of viral DNA by PCR analysis. Results: DNA of polyomaviruses was found in oligodendrogliomas (66.7% of cases), oligodendroastrocytomas (66.7% of cases) and glioblastomas (40% of cases). In metastatic tumors viral infection had not been detected. No correlation between the grade of brain tumors and infection with polyomavirus DNA has been revealed. Conclusion: our data point to importance of investigation of the brain tumors and cerebrospinal fluid for determination of viral infection.
