EFFECT OF 2’-DEOXYISOGUANOSINE ON THE GROWTH OF HUMAN TUMOR AND NORMAL CELL LINES
Radoslawa Nowak1,*, Maria Sawardo-Rochowska2, Zygmunt Kazimierczuk3, Roman J. Nowak4
1Department of Molecular Biology, The Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland 2Department of Immunology, The Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland 3Institute of Chemistry, Agricultural University, and Laboratory of Experimental Pharmacology, Polish Academy of Sciences Medical Research Center, Warsaw, Poland 4Institute of Experimental Physics, Warsaw University, Warsaw, Poland
Abstract. Aim: To test the effect of 2’-deoxyisoguanosine as a specific substrate of HIV reverse transcriptase on the growth of cultured normal and cancer cells. Methods: The effect of 2’-deoxyisoguanosine on the growth of cells of two normal (telomerase-negative) and five cancer cell lines have been tested. Cell viability was assessed by MTT assay. Results: We have found that this nucleoside analogue has low potency and specificity in inhibiting tumor cell growth. IC50 ranged from 0.5 mM to 2 mM for tumor cells, and from 1 mM to more than 4 mM for normal cells. Conclusions: 2’-deoxyisoguanosine has low potency and specificity in inhibiting tumor cell growth, similar to other telomerase inhibitors.
