Vol. 25, No. 4, 2003 (December)
	Content

IMMUNOHISTOCHEMICAL ANALYSIS OF p70S6 KINASE a IN HUMAN THYROID TISSUE UPON PATHOLOGY

V.V. Lyzogubov¹, V.S. Usenko^1,*, Yu.S. Khojaenko¹, D.I. Lytvyn², M.A. Soldatkina², N.V. Rodnin³, V.V. Filonenko³, P.V. Pogribniy²

¹Morphological Laboratory “BIONTEC”, Dniepropetrovsk, Ukraine
²R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kyiv, Ukraine
³Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, Ukraine

Abstract. Aim: to evaluate p70S6 kinase a (p70S6K a) content in epithelial cells of thyroid gland in normal state and upon pathology. Methods: the study was carried out by quantitative immunohistochemical analysis with the use of anti-p70S6K a-MoAbs, image computer analysis and statistical analysis on autopsy samples of thyroid gland in normal state (3 cases), and on surgical samples of diffuse toxic goiter (3 cases) and papillary thyroid carcinoma (6 cases). Results: normal state of thyroid gland is characterised by low content of p70S6K a. However, upon hyperthyreosis and papillary thyroid cancer, p70S6K a content is 4-folds and 9-folds higher respectively. Conclusion: hyperfunction and carcinogenesis of thyroid gland is accompanied by increase of the content of p70S6K a in thyroid epithelial cells.

Key Words: p70S6 kinase a, thyroid gland, diffuse toxic goiter, papillary carcinoma, immunohistochemistry.

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