GROWTH-INHIBITORY EFFECT OF EGFR INHIBITOR AG1487 ON HUMAN TONGUE CANCER CELLS TCA8113
Xin-guang Han*, Bo-gui Wen
Laboratory of Tumor Molecular Biology, Department of Pathology, Medical College, Shantou University, Shantou 515041, China
Abstract. Objective: the aim of this study was to investigate the growth inhibitory effect of epidermal growth factor receptor (EGFR) inhibitor AG1487 on human tongue cancer cells. Methods: the cultured Tca8113cells were treated with different concentrations of phosphotyrosine kinase inhibitor AG1487, then MTT method, Western blot, flow cytometry and DNA ladder analysis were employed to investigate the inhibitory effect of AG1487 on cell growth, expression of total ERK1/2 and activated ERK1/2, and apoptosis of Tca8113 cells. Results: when the concentration of AG1487 has been increased, growth of cells was gradually inhibited, and expression of activated ERK1/2 was decreased. When the concentration of AG1487 reached 200 nM, the expression of activated ERK1/2 was nearly abolished, however there was no significant change of that of total ERK1/2. Apoptosis of Tca8113 cells induced by AG1487 has dose-dependent and time-dependent character. Conclusion: treatment of tumor cells with EGFR inhibitor AG1487 leads to significant growth inhibition and apoptosis.
