Vol. 25, No. 4, 2003 (December)
	Content

EXPRESSION OF a- AND b-ISOFORMS OF р70S6 KINASE IN HUMAN ENDOMETRIAL TUMORS

D.I. Lytvyn¹, T.M. Dudchenko¹, V.V. Lyzogubov², V.S. Usenko², S.V. Nespryadko³, A.B. Vinnitskaya³, L.I. Vorobyova³, S.S. Pal’chevskiy⁴, V.V. Filonenko⁴, P.V. Pogrebnoy^1,*

¹R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
²Morphological Laboratory “Biontec”, Dniepropetrovsk, Ukraine
³Institute of Oncology, AMS of Ukraine, Kyiv, Ukraine
⁴Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, Ukraine

Abstract. Aim: to investigate the character of expression of p70S6Кa and b in human endometrial tumors and conventually normal tissues surrounding tumor. Methods: the expression of p70S6Кa and b has been studied in 16 samples of human uterine adenocarcinoma, 2 samples of adenosquamous cancer, and blood sera samples of mentioned patients and healthy donors by Western blot analysis, ELISA, immunohistochemistry, RT-PCR. Results: by Western blotting, hyperexpression of p70S6Кa and p70S6Кb was revealed in 56 and 67% cases of uterine adenocarcinoma compared to normal tissues. p70S6Кb mRNAs level was higher than that of p70S6Кa in tumor tissues and correlates with expression of kinase on protein level. Immunohistochemical analysis has shown the increased p70S6Кa expression in tumor stroma, endothelium of the vessels both of tumors and adjusting non-tumor tissues. Conclusion: p70S6Кa and b isoforms may play alternative roles in malignant transformation and progression of human endometrial tumors.

Key Words: p70S6Ka, p70S6Kb, expression, tumor, adenocarcinoma, uterus.

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