Vol. 25, No. 4, 2003 (December)
	Content

INFLUENCE OF ІFN-a2b, TGF-b1 AND SOME CHEMOTHERAPEUTIC AGENTS ON THE PROLIFERATION OF ENDOTHELIAL CELLS

М.І. Lomnytska^1,2,*, N.A. Volodko¹, S. Souchelnytskyi², B.T. Bilynskyi¹

¹Department of Oncology and Medical Radiology, Lviv National Medical University, Ukraine
²Ludwig Institute for Cancer Research, Uppsala branch, Sweden

Abstract. Aim: to study in vitro the antiproliferative influence of interferon-a2b (ІFN-a2b), transforming growth factor-b₁ (TGF-b₁), cys-platinum, cyclophosphamide, and paclitaxel on the of human microvascular endothelial cells for the development of approaches of the antiangiogenic therapy of malignancies. Methods: [³H]-methylthymidine incorporation into human microvascular endothelial derma-derived cells (HMVECd) treated with ІFN-a2b, TGF-b₁, cysplatinum, cyclophosphamide, and paclitaxel have been studied. Results: studied cytokines and drugs inhibited proliferation of HMVECd cells at low concentrations (ІFN-a2b — 50.0 and 100.0 IU/ml; TGF-b₁ — 5.0 ng/ml; cysplatinum — 0.5 mg/ml; paclitaxel — 10.0 mg/ml; cyclophosphamide — 50.0 mg/ml). Conclusion: low concentrations of ІFN-a2b, ТGF-b₁, cysplatinum, cyclophosphamide, аnd paclitaxel inhibited proliferation of cultured endothelial cells, suggesting a possibility of low-dose antiangiogenic therapy in the long-term (“metronomic”) regimen.

Key Words: angiogenesis, antiangiogenic therapy, interferon-a2b, transforming growth factor-b₁, cys-platinum, cyclophosphamide, paclitaxel, human microvascular endothelial cells.

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