R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine Resume. For preparation of recombinant human beta defensin-2 (hBD-2), the expression of the peptide fused with GST in bacterial system was chosen. hBD-2 cDNA fragment was cloned in pGEX-2T vector and the construct was introduced into E.coli XL-Gold cells. Fusion protein was purified by affine chromatography on GST-sepharose, and underwent proteolysis with trombin. Active recombinant hBD-2 was purified from the proteolytic mixture by the ion exchange chromatography. Key words: hBD-2, cloning, expression, fusion protein.
Abstract. For preparation of recombinant human beta defensin-2 (hBD-2), the expression of the peptide fused with GST in bacterial system was chosen. hBD-2 cDNA fragment was cloned in pGEX-2T vector and the construct was introduced into E.coli XL-Gold cells. Fusion protein was purified by affine chromatography on GST-sepharose, and underwent proteolysis with trombin. Active recombinant hBD-2 was purified from the proteolytic mixture by the ion exchange chromatography.
