DETECTION OF p53 DELETIONS USING FISH TECHNIQUE IN THE HL-60, DAUDI, RAJI AND K-562 CELL LINES
E. Konac, M.A. Ergun, F.I. Sahin, A. Ekmekci*
Gazi University, Besevler 06500, Ankara, Turkey Resume. The work is aimed on the analysis of chromosome 17 centromere and p53 gene-specific signals within interphase and metaphase, determination of p53 deletion ratios and the detection of the cell-cycle progress. The loss of heterozygosity (LOH) has been investigated using FISH method in 4 cell lines (HL-60, Daudi, Raji and K-562) and peripheral blood samples from 3 Burkitt’s lymphoma patients and 2 healthy donors. It is concluded that FISH method may be used in aneuploidy as well as in detection of p53 deletions and mutations. Key words: p53 mutation, cell line, loss of heterozygosity, FISH.
Abstract. The work is aimed on the analysis of chromosome 17 centromere and p53 gene-specific signals within interphase and metaphase, determination of p53 deletion ratios and the detection of the cell-cycle progress. The loss of heterozygosity (LOH) has been investigated using FISH method in 4 cell lines (HL-60, Daudi, Raji and K-562) and peripheral blood samples from 3 Burkitt’s lymphoma patients and 2 healthy donors. It is concluded that FISH method may be used in aneuploidy as well as in detection of p53 deletions and mutations.
Key Words:p53 mutation, cell line, loss of heterozygosity, FISH.
