Vol. 25, No. 1, 2003 (March)
	Content

APPLICATION OF FISH TECHNIQUE FOR CYTOGENETIC ANALYSIS IN CHRONIC MYELOID LEUKEMIA PATIENTS

S. Kasakyan¹, G. Tachdjian², E. Movsessyan³, S. Dagbhashyan³, L. Muradyan³,

A. Aboura², A. Hovannisyan³, A. Muradyan¹, H. Mkrtchyan¹, R. Arutyunyan^1,*
¹Yerevan State University, Yerevan 375025, Armenia
²Antoine Beclere Hospital, Clamart 92141, France
³Hematological Center of Ministry of Health, Yerevan 375014, Armenia
Resume. The detection of cytogenetic anomalies is important for the diagnosis and prognosis of hematological malignant diseases. Karyotypes of 32 Armenian patients with chronic myeloid leukemia have been studied for the presence of Philadelphia (Ph) chromosome resulting from translocation t(9;22)(q34;q11) by means of conventional karyotype analysis (CK) and fluorescence in situ hybridization (FISH) in the bone marrow and/or peripheral blood specimens. In our study application of metaphase or interphase FISH in chronic myeloid leukemia patients permitted to confirm and improve the results of conventional cytogenetic analysis. Key words: cytogeneti

Abstract. The detection of cytogenetic anomalies is important for the diagnosis and prognosis of hematological malignant diseases. Karyotypes of 32 Armenian patients with chronic myeloid leukemia have been studied for the presence of Philadelphia (Ph) chromosome resulting from translocation t(9;22)(q34;q11) by means of conventional karyotype analysis (CK) and fluorescence in situ hybridization (FISH) in the bone marrow and/or peripheral blood specimens. In our study application of metaphase or interphase FISH in chronic myeloid leukemia patients permitted to confirm and improve the results of conventional cytogenetic analysis.

Key Words: cytogenetics, chronic myeloid leukemia, Philadelphia chromosome, FISH.

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