Vol. 24, No. 2, 2002 (June)
	Content

CHAPERON HSP-60 AS AUTOANTIGEN IN DEVELOPMENT OF DYSHORMONAL BREAST DISEASES

O.G. Vigontina¹, O.A. Efimenko², L.F. Yakovenko¹, R.G. Kiyamova¹, V.V. Filonenko¹, I.T. Gout³, N.V. Ros’ ², N.V. Kosey², T.F. Tatarchuk², L.L. Sidorik^1,*, G.Kh. Matsuka¹

¹Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, Ukraine
²Institute of Pediatrics, Obstetrics and Ginecology, AMS of Ukraine, Kyiv, Ukraine
³Ludwig Institute for Cancer Research, London, United Kingdom

Abstract. Recombinant Hsp-60 chaperon was isolated from the lyzate of E.coli cells transformed with plasmid pT-GroESL, purified by gel-filtration and ion exchange chromatography and used as antigen in the study of autoimmune processes in patients with dyshormonal breast diseases (DBD). It has been shown that the sera of 80% of DBD patients were anti-Hsp-60 positive and the level of anti-Hsp-60 antibodies in the sera of DBD patients was significantly higher (p < 0.05) that that in the sera of healthy donors. The highest levels of anti-Hsp-60 antibodies were registered in the sera of the DBD patients with simultaneous cancer processes.

Key Words: chaperon, dyshormonal breast diseases, breast cancer.

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